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The American Biology Teacher

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  • Inquiry & Investigations
Using Environmental DNA to Connect Lab Science with Field Practice
James McNeil, Anneke DeLuycker, Sarah Putman
The American Biology Teacher, Vol. 80 No. 4, April 2018; (pp. 285-289) DOI: 10.1525/abt.2018.80.4.285
James McNeil
JAMES McNEIL (jmcneil@gmu.edu) is an Assistant Professor, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
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Anneke DeLuycker
ANNEKE DeLUYCKER (adeluyck@gmu.edu) is an Assistant Professor, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
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Sarah Putman
SARAH PUTMAN (sputman@gmu.edu) is a Laboratory Manager, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
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Abstract

Experiential learning helps students make connections between different skill sets and allows them to engage in a deeper level of inquiry. To enhance the connection between field and laboratory practice for undergraduate students in our wildlife ecology curriculum, we developed an exercise using environmental DNA (eDNA) analysis. eDNA sampling involves extracting and amplifying the DNA from specific organisms from an environmental sample, rather than from the organisms themselves, and has been rapidly adopted by conservation practitioners around the world. In our activity, students collect water samples from a local pond and process them to detect the presence of American bullfrogs. Practicing this procedure not only introduces them to professional skills they may utilize in their careers, but also helps create context for how laboratory science and field work support each other and can be used to connect to larger issues of conservation, environmental studies, or ecology.

  • eDNA
  • amphibians
  • biodiversity sampling
  • experiential learning
  • © 2018 National Association of Biology Teachers. All rights reserved. Please direct all requests for permission to photocopy or reproduce article content through the University of California Press's Reprints and Permissions web page, www.ucpress.edu/journals.php?p=reprints.
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Vol. 80 No. 4, April 2018

The American Biology Teacher: 80 (4)
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Using Environmental DNA to Connect Lab Science with Field Practice
James McNeil, Anneke DeLuycker, Sarah Putman
The American Biology Teacher, Vol. 80 No. 4, April 2018; (pp. 285-289) DOI: 10.1525/abt.2018.80.4.285
James McNeil
JAMES McNEIL (jmcneil@gmu.edu) is an Assistant Professor, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
  • Find this author on Google Scholar
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: jmcneil@gmu.edu
Anneke DeLuycker
ANNEKE DeLUYCKER (adeluyck@gmu.edu) is an Assistant Professor, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
  • Find this author on Google Scholar
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: adeluyck@gmu.edu
Sarah Putman
SARAH PUTMAN (sputman@gmu.edu) is a Laboratory Manager, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
  • Find this author on Google Scholar
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: sputman@gmu.edu

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Using Environmental DNA to Connect Lab Science with Field Practice
James McNeil, Anneke DeLuycker, Sarah Putman
The American Biology Teacher, Vol. 80 No. 4, April 2018; (pp. 285-289) DOI: 10.1525/abt.2018.80.4.285
James McNeil
JAMES McNEIL (jmcneil@gmu.edu) is an Assistant Professor, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
  • Find this author on Google Scholar
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: jmcneil@gmu.edu
Anneke DeLuycker
ANNEKE DeLUYCKER (adeluyck@gmu.edu) is an Assistant Professor, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
  • Find this author on Google Scholar
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: adeluyck@gmu.edu
Sarah Putman
SARAH PUTMAN (sputman@gmu.edu) is a Laboratory Manager, at the Smithsonian-Mason School of Conservation, 184 Farm Road, Front Royal, VA 22630, USA.
  • Find this author on Google Scholar
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: sputman@gmu.edu
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